911勛圖

Synopsis

The gold standard in chemical synthesis of nucleic acids���DNA and RNA���typically yields only one oligonucleotide sequence per run, followed by labor-intensive downstream analysis. This double bottleneck is partially addressed by microarray technology, which enables rapid, discrete analysis of thousands of pre-synthesized oligonucleotides. However, these oligonucleotides are still produced in a low-throughput manner and then immobilized on individual surface-bound features, forming structured libraries, or microarrays, that can be analyzed simultaneously. 繒^, 簡

To overcome the bottleneck of synthesis itself, spatially controlled in situ synthesis has been developed. Here, photolithographic synthesis stands out as the most robust���and currently the only high-throughput���approach for fabricating both DNA and RNA oligonucleotides. 簡^, 糧

This talk will showcase how we advanced the photolithographic synthesis of RNA and how it can be harnessed to generate highly reproducible, high-density oligonucleotide microarrays. It will cover applications in systematic RNA aptamer screening 簡, in probing sequence-dependence of fluorescence dyes ���^, ���, and in storage of digital data through complementary hybridization. 糧^, ���

1. J. Lietard, M. M. Somoza, Spotting, Transcription and In Situ Synthesis: Three Routes for the Fabrication of RNA Microarrays. CSBJ 17, 862-68, (2019). DOI: 10.1016/j.csbj.2019.06.004.
2. T. Kekic et al., Accelerated, high-quality photolithographic synthesis of RNA microarrays in situ. Sci. Adv.10, eado6762, (2024). DOI:10.1126/sciadv.ado6762
3. T. Kekic, J. Lietard, A Canvas of Spatially Arranged DNA Strands that Can Produce 24-bit Color Depth. JACS 145, 22293-97, (2023). DOI: 10.1021/jacs.3c06500.
4. T. Kekic, J. Lietard, An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting. Nanoscale 14,17528-33, (2022). DOI: 10.1039/d2nr05269e.
5. T. Kekic, J. Lietard, Sequence-dependence of Cy3 and Cy5 dyes in 3妏 terminally-labeled single-stranded DNA, Sci. Rep. 12,14803, (2022). DOI: 10.1038/s41598-022-19069-9